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KMID : 0364819730110020079
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Korean Journal of Microbiology
1973 Volume.11 No. 2 p.79 ~ p.88
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Effect of Co^60 γ-ray on Salmonella typhi, Ty2 cell
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이강순
민봉희/장정순
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Abstract
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In order to investigate the effect of gamma radiation on Salmonella typhi, Ty2, the components of amino acids, proteins, carbohydrates and lipids in irradiated cells were compared with those in unirradiated control cells respectively. The results obtained were as follows ; 1) The inactivation curves of Salmonella typhi with Co^60 γ-ray irradiation were exponential over a wide range to the irradiated doses. 2) Dose for the inactivation factor of 10^8 was 94.0 Krad in physiological saline or in phosphate buffered saline, 104.2 Krad in nutrient broth, 220.4Krad in frozen state, 552.0 Krad in dried state, 88.3 Krad in the abundance of oxygen and 188.0 Krad in the deficience of oxygen, respectively. 3) Five consecutive irradiation of Salmonella typhi suspension at the dose of 90 Krad gave no additional increase in resistance. 4) Even at the smallest dose of 500 Krad, compositions of amino acids, proteins, carbohydrates, and lipids were more or less decreased and the distinct banding patterns were also lost possibly due to degradation of the protein molecules.
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KEYWORD
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